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KMID : 0368419990420010075
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Journal of Plant Biology
1999 Volume.42 No. 1 p.75 ~ p.80
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A cDNA Clone from Broccoli(Brassica oleracea) for a New Type of Oleosin
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Suh, Mi Chung
Kim, Young Soon/Hong, Choo Bong/Nam, Sang Hyeon/Cho, Young Hwan
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Abstract
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From a differential screening of a cDNA library for broccoli anther, a cDNA clone for oleosin was isolated and named BASC1. BASC1 did not contain the ammo-terminus but probably coded for most of the protein. The open reading frame extending for 212 amino acids had a very long hydrophobic region flanked by amphiphatic regions, the typical pattern for oleosins. In the long hydrophobic region, BASC1 showed strong homologies to the previously reported oleosins. To the carboxy-terminus from the center of ORF, BASC1 is unique in having a region of hydrophilicity in the middle of the amphiphatic stretch. Transcripts of BASC1 were detected from the anther of uninucleate and binucleate pollens, and from the tapetum and pollen.
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KEYWORD
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